INVESTIGATION OF BIOFILM FORMATION IN COAGULASE-NEGATIVE STAPHYLOCOCCI ISOLATED FROM PLATELET CONCENTRATE BAGS

Platelet Concentrates (PCs) are the blood components with the highest rate of bacterial contamination, and coagulase-negative staphylococci (CoNS) are the most frequently isolated contaminants. This study investigated the biofilm formation of 16 contaminated units out of 691 PCs tested by phenotypic and genotypic methods. Adhesion in Borosilicate Tube (ABT) and Congo Red Agar (CRA) tests were used to assess the presence of biofilm. The presence of icaADC genes was assessed by means of the Polymerase Chain Reaction (PCR) technique. With Vitek(r)2, Staphylococcus haemolyticus was considered the most prevalent CoNS (31.25%). The CRA characterized 43.8% as probable biofilm producers, and for the ABT test, 37.5%. The icaADC genes were identified in seven samples by the PCR. The ABT technique showed 85.7% sensitivity and 100% specificity when compared to the reference method (PCR), and presented strong agreement (k = 0.8). This study shows that species identified as PCs contaminants are considered inhabitants of the normal skin flora and they might become important pathogens. The results also lead to the recommendation of ABT use in laboratory routine for detecting biofilm in CoNS contaminants of PCs.

Fasciite necrosante por Aeromonas hydrophila e Staphylococcus epidermidis ­ relato de caso / Necrotizing fasciitis caused by Aeromonas hydrophil a and Staphylococcus epidermidis ­ case report

Introdução: Fasciite necrosante (FN) é uma infecção rara dos tecidos subcutâneos e fáscia superficial, geralmente confundida com infecção benigna. Entretanto, apresenta enorme potencial para o desenvolvimento de complicações graves que contribuem para os elevados índices de mortalidade. Objetivos: Descrever um caso de FN polimicrobiana ocasionada por Aeromonas hydrophila e Staphylococcus epidermidis em paciente portador de síndrome da imunodeficiência adquirida, hepatite C e diabetes mellitus. Métodos: Analisaram-se dados de prontuário e resultados de exames laboratoriais de paciente internado no Hospital Universitário de Santa Maria, Santa Maria, Rio Grande do Sul. Resultados: Paciente do sexo masculino, 47 anos, com relato de fratura exposta em membro inferior esquerdo, desenvolvendo infecção no ferimento. Após desbridamento de tecido desvitalizado, identificaram-se A. hydrophila e S. epidermidis. Paciente continua em tratamento e aguarda cirurgia para enxerto. Conclusões: A FN é uma enfermidade rara que merece toda a atenção médica, pois a identificação e tratamento precoces são fundamentais para a recuperação física do paciente.

Introduction: Necrotizing fasciitis (NF) is a rare infection of the subcutaneous tissue and superficial fascia, usually confused with benign infection. However, it has tremendous potential for the development of serious complications which contribute to the high mortality rates. Objectives: To describe a case of FN caused by Aeromonas hydrophila polymicrobial and Staphylococcus epidermidis in patient immunodeficiency syndrome carrier acquired hepatitis C and diabetes mellitus. Methods: We analyzed data from medical records and laboratory test results of inpatient at the University Hospital of Santa Maria, Santa Maria, Rio Grande do Sul. Results: Male patient, 47 years of age, with compound fracture reporting in the left lower limb, developing infection in the wound. After debridement of devitalized tissue, A. hydrophila and S. epidermidis were identified. Patient continues processing and waits for grafting surgery. Conclusions: The FN is a rare disease that deserves medical attention, for the early identification and treatment are essential for the physical recovery of the patient.

Fasciite necrosante por Streptococcus agalactiae em paciente diabética ? relato de caso / Necrotizing fasciitis caused by Streptococcus agalactiae in a diabetic patient ? case report

Objetivos: Descrever um caso de fasciite necrosante e choque séptico ocasionado por Streptococcus agalactiae, que acometeu uma paciente com diabetes mellitus. Esta análise foi realizada através dos dados do prontuário e resultados de exames laboratoriais da paciente, que estava internada no Hospital Universitário de Santa Maria, em Santa Maria, Rio Grande do Sul.Descrição do caso: Paciente do gênero feminino, 73 anos, diagnosticada com diabetes mellitus e cirrose micronodular, foi internada com suspeita de septicemia, simultânea a uma infecção no membro inferior esquerdo, cuja hipótese diagnóstica inicial foi de celulite. Pelas características do quadro clínico, foi feito o diagnóstico de fasciite necrosante. Hemoculturas de dois sítios diferentes positivaram para S. agalactiae. A paciente foi a óbito por choque séptico.Conclusões: O relato deste caso enfatiza a gravidade da fasciite necrosante, que pode ocorrer principalmente em pacientes portadores de fatores predisponentes como diabetes mellitus e cirrose.

Aims: To report an unusual case of necrotizing fasciitis and septic shock caused by Streptococcus agalactiae, which affected a patient with diabetes mellitus. This analysis was performed using data from medical records and laboratory tests results, who was admitted to the University Hospital of Santa Maria, in Santa Maria, Rio Grande do Sul, Brazil.Case description: Female patient, 73 years old, diagnosed with diabetes mellitus and micronodular cirrhosis, was admitted with suspected septicemia simultaneous with an infection in the left lower limb, which initial diagnostic hypothesis was cellulitis. Based on characteristics of the clinical picture, the diagnosis of necrotizing fasciitis was done. Blood cultures from two different sites were positive for S. agalactiae. The patient died with septic shock.Conclusions: This case report emphasizes the severity of necrotizing fasciitis, which may occur especially in patients with predisposing factors such as diabetes mellitus and cirrhosis.